Application
Western blot analysis of Troponin C expression in non-transfected 293T: (A), human Troponin C transfected 293T: (B) and SJRH30 (C) whole cell lysates.Application
Western blot analysis of Troponin C expression in non-transfected: (A) and human Troponin C transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Western blot analysis of Troponin C expression in L6 whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Product Name | Troponin C Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between actin and myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator), and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has 2 isoforms: fast and slow. Fast troponin C has two calcium binding sites while slow/cardiac troponin C has a single calcium binding site. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of fast skeletal and, to a lesser extent, slow skeletal/cardiac isoforms of Troponin C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-160 of Troponin C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-160 of Troponin C of human origin |
| Entrez Gene | 7136 |
|---|
Application
Western blot analysis of Troponin C expression in non-transfected 293T: (A), human Troponin C transfected 293T: (B) and SJRH30 (C) whole cell lysates.
Application
Western blot analysis of Troponin C expression in non-transfected: (A) and human Troponin C transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Western blot analysis of Troponin C expression in L6 whole cell lysate (A) and rat skeletal muscle tissue extract (B).| Application Notes | recommended for detection of fast skeletal and, to a lesser extent, slow skeletal/cardiac isoforms of Troponin C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
