Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Trk B expression in rat brain tissue extract.Application
Western blot analysis of human recombinant Trk B.| Product Name | Trk B Antibody (794) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of the Trk B splice variants L1 and L10 of mouse origin, Trk B gp145 of rat origin and Trk B of human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with Trk B gp95 of rat origin, Trk B-T1 or Trk B T-Shc of human origin, Trk A or Trk C also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of Trk B of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of Trk B of mouse origin |
| Entrez Gene | 4915 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Trk B expression in rat brain tissue extract.
Application
Western blot analysis of human recombinant Trk B.| Application Notes | recommended for detection of the Trk B splice variants L1 and L10 of mouse origin, Trk B gp145 of rat origin and Trk B of human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with Trk B gp95 of rat origin, Trk B-T1 or Trk B T-Shc of human origin, Trk A or Trk C also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
