Application
Western blot analysis of RAGE expression in non-transfected: (A) and human RAGE transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A549 cells showing membrane staining.Application
Western blot analysis of RAGE expression in mouse lung extract.| Product Name | RAGE Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RAGE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RAGE of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of RAGE of human origin |
| Entrez Gene | 177 |
|---|
Application
Western blot analysis of RAGE expression in non-transfected: (A) and human RAGE transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing membrane staining.
Application
Western blot analysis of RAGE expression in mouse lung extract.| Application Notes | recommended for detection of RAGE of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
