Application
Western blot analysis of Gab 2 expression in K-562 (A), MCP-5 (B), OV-90 (C), ES-2 (D), A549 (E) and F9 (F) whole cell lysates.| Product Name | Gab 2 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter protein such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity (1-3,8). These are immediate substrates of receptor tyrosine kinase activity and serve to link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate (4-7). Shc and Gab 1 also participate in insulin signaling by linking the insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) and Sos independently of IRS-1. The Gap 1 related protein, Gab 2, associates with SH2 domain-containing proteins, such as SHP2, and it is involved in a novel pathway for cytokine-induced gene activation (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gab 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-320 mapping near the N-terminus of Gab 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-320 mapping near the N-terminus of Gab 2 of human origin |
| Entrez Gene | 9846 |
|---|
Application
Western blot analysis of Gab 2 expression in K-562 (A), MCP-5 (B), OV-90 (C), ES-2 (D), A549 (E) and F9 (F) whole cell lysates.| Application Notes | recommended for detection of Gab 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
