Application
Western blot analysis of Gα q/11 expression in human platelet whole cell lysate.Application
Western blot analysis of Gα 14 expression in non-transfected: (A) and human Gα 14 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Gα q/11 expression in Jurkat (A), HeLa (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of Gα q/11 expression in mouse brain extract.| Product Name | Gα q/11 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gå subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gq class includes Gå 15,Gå 14, Gå 11 and Gå q, two of which, Gå 11 and Gå q are abundant in brain and lung and present at lower levels in a variety of tissues (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of G&alpha q and G&alpha 11 of mammalian origin by WB, IP, IF and ELISA may cross-react with G&alpha 14 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a domain common to G&alpha 11 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within a domain common to Gα 11 of mouse origin |
| Entrez Gene | 14672 |
|---|
Application
Western blot analysis of Gα q/11 expression in human platelet whole cell lysate.
Application
Western blot analysis of Gα 14 expression in non-transfected: (A) and human Gα 14 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Gα q/11 expression in Jurkat (A), HeLa (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of Gα q/11 expression in mouse brain extract.| Application Notes | recommended for detection of G&alpha q and G&alpha 11 of mammalian origin by WB, IP, IF and ELISA may cross-react with G&alpha 14 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
