Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear staining of squamous epithelial cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining in exocrine pancreas cells and nuclear and cytoplasmic in islet cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of I2PP2A expression in Ramos whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing nuclear localization in selected cells.Application
Immunofluorescence staining of methanol-fixed Ramos cells showing nuclear and cytoplasmic staining.Application
Western blot analysis of human recombinant I2PP2A fusion protein.| Product Name | I2PP2A Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Protein phosphatase 2A PP2A is a major mammalian protein serine/threonine phosphatase that regulates diverse cellular processes. Inhibitor 1 of PP2A (I1PP2A) and inhibitor 2 of PP2A (I2PP2A), which share large sequence similarity, are heat-stable protein inhibitors of the cellular phosphatase activity of PP2A. I1PP2A and I2PP2A were initially characterized as putative HLA class II associated proteins Phap I and Phap II. These inhibitor proteins act noncompetitively to selectively inhibit PP2A, but do not affect the phosphatase activity of the related proteins PP1, PP2B and PP2C. The I1PP2A protein is localized to both the cytoplasm and the nucleus. In contrast, I2PP2A is located predominantly in the nucleus and is highly expressed in Wilms’ tumor cells. Transient expression of I2PP2A in HEK-293 cells leads to an increase in the DNA binding activity of the proto-oncogene c-Jun. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of I2PP2A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-120 mapping at the N-terminus of I2PP2A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-120 mapping at the N-terminus of I2PP2A of human origin |
| Entrez Gene | 6418 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nuclear staining of squamous epithelial cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining in exocrine pancreas cells and nuclear and cytoplasmic in islet cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of I2PP2A expression in Ramos whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tumor showing nuclear localization in selected cells.
Application
Immunofluorescence staining of methanol-fixed Ramos cells showing nuclear and cytoplasmic staining.
Application
Western blot analysis of human recombinant I2PP2A fusion protein.| Application Notes | recommended for detection of I2PP2A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
