Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing membrane, cytoplasmic and nuclear staining of glandular and lymphoid cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Notch 3 expression in BC3H1 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and nuclear localization.| Product Name | Notch 3 Antibody (M-134) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2107-2240 mapping near the C-terminus of Notch 3 of mouse origin |
| Modification Notes | The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four Notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The Notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions. Normal Delta expression is restricted to the adrenal gland and placenta. Delta expression has also been found in neuroendocrine tumors such as neuroblastomas and pheochromocytomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Notch 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2107-2240 mapping near the C-terminus of Notch 3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2107-2240 mapping near the C-terminus of Notch 3 of mouse origin |
| Entrez Gene | 4854 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing membrane, cytoplasmic and nuclear staining of glandular and lymphoid cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Notch 3 expression in BC3H1 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane and nuclear localization.| Application Notes | recommended for detection of Notch 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
