Application
Western blot analysis of Annexin V expression in non-transfected: (A) and mouse Annexin V transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Annexin V expression in non-transfected 293T: (A), human Annexin V transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Annexin V expression in non-transfected 293T: (A), human Annexin V transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Annexin V expression in non-transfected control (A) and Annexin V siRNA transfected (B) HeLa cells. Blot probed with Annexin V (FL-319): . β Tubulin (D-10): used as specificity and loading control.Application
Western blot analysis of Annexin V expression in HeLa whole cell lysate.| Product Name | Annexin V Antibody (FL-319) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-term-inal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Annexin V and, to a lesser extent, Annexin IV and Annexin VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-319 representing full length Annexin V of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-319 representing full length Annexin V of human origin |
| Entrez Gene | 308 |
|---|
Application
Western blot analysis of Annexin V expression in non-transfected: (A) and mouse Annexin V transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Annexin V expression in non-transfected 293T: (A), human Annexin V transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Annexin V expression in non-transfected 293T: (A), human Annexin V transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Annexin V expression in non-transfected control (A) and Annexin V siRNA transfected (B) HeLa cells. Blot probed with Annexin V (FL-319): . β Tubulin (D-10): used as specificity and loading control.
Application
Western blot analysis of Annexin V expression in HeLa whole cell lysate.| Application Notes | recommended for detection of Annexin V and, to a lesser extent, Annexin IV and Annexin VI of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
