Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of Anamorsin expression in non-transfected 293T: (A), human Anamorsin transfected 293T: (B), MIA PaCa-2 (C) and HeLa (D) whole cell lysates.Application
Western blot analysis of Anamorsin expression in HeLa (A), COLO 320DM (B), T24 (C) and Hep G2 (D) whole cell lysates and mouse testis tissue extract (E).Application
Western blot analysis of Anamorsin expression in non-transfected 293T: (A), mouse Anamorsin transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Anamorsin Antibody (FL-312) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The name of the protein Anamorsin, also designated cytokine-induced apoptosis inhibitor 1 (CIAPIN1), comes from the Latin term “ana-mors-in”, meaning “anti-death molecule”. During hematopoiesis, Anamorsin is crucial for mediating the anti-apoptotic effects of various cytokines. It is a ubiquitously expressed protein, and when it is overexpressed, it confers apoptotic resistance. Anamorsin is primarily expressed in the cytoplasm of liver, pancreas and heart tissue cells and does not show any homology to known apoptosis regulatory molecules of the Bcl-2 or CASP families, or to signal transduction molecules. Anamorsin expression in mouse cells confers resistance to apoptosis caused by IL-3 (interleukin-3) deprivation. Studies demonstrate that the addition of growth factors, such as EPO (erythropoietin), SCF (stem cell factor), TPO (thrombopoietin) or IL-3, all of which depend on RAS signaling, induce dose-dependent expression of Anamorsin in mouse cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Anamorsin isoforms 1,2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-312 representing full length Anamorsin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-312 representing full length Anamorsin of human origin |
| Entrez Gene | 57019 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Anamorsin expression in non-transfected 293T: (A), human Anamorsin transfected 293T: (B), MIA PaCa-2 (C) and HeLa (D) whole cell lysates.
Application
Western blot analysis of Anamorsin expression in HeLa (A), COLO 320DM (B), T24 (C) and Hep G2 (D) whole cell lysates and mouse testis tissue extract (E).
Application
Western blot analysis of Anamorsin expression in non-transfected 293T: (A), mouse Anamorsin transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Anamorsin isoforms 1,2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
