Application
Western blot analysis of V-ATPase H expression in non-transfected: (A) and mouse V-ATPase H transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of V-ATPase H expression in mouse brain tissue extract.| Product Name | V-ATPase H Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase (1). Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain (1). Like F-ATPase, V-ATPase most likely operates through a rotary mechanism (1). The H subunit of V-ATPase, also designated SDF is comprised of two polypeptides derived from the same gene (2-4). This regulatory subunit plays a critical role in the functional coupling of ATP hydrolysis activity to proton transport in the V-ATPase pump (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of V-ATPase H of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 184-483 mapping at the C-terminus of V-ATPase H of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 184-483 mapping at the C-terminus of V-ATPase H of human origin |
| Entrez Gene | 51606 |
|---|
Application
Western blot analysis of V-ATPase H expression in non-transfected: (A) and mouse V-ATPase H transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of V-ATPase H expression in mouse brain tissue extract.| Application Notes | recommended for detection of V-ATPase H of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
