Application
Western blot analysis of SCF expression in non-transfected: (A) and mouse SCF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SCF expression in HeLa (A), SW480 (B) and A549 (C) whole cell lysates.| Product Name | SCF Antibody (H-189) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SCF of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-214 representing the extracellular domain of stem cell factor (SCF) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-214 representing the extracellular domain of stem cell factor (SCF) of human origin |
| Entrez Gene | 4254 |
|---|
Application
Western blot analysis of SCF expression in non-transfected: (A) and mouse SCF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SCF expression in HeLa (A), SW480 (B) and A549 (C) whole cell lysates.| Application Notes | recommended for detection of SCF of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
