Application
Western blot analysis of MIF expression in AML-193 whole cell lysate.Application
Western blot analysis of MIF expression in Jurkat whole cell lysate.Application
Western blot analysis of MIF expression in non-transfected 293T: (A), human MIF transfected 293T: (B) and Y79 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic and extracellular localization.Application
Western blot analysis of MIF expression in Y79 (A), U-937 (B) and HL-60 (C) whole cell lysates.Application
Western blot analysis of MIF expression in U-937 (A) and HL-60 (B) whole cell lysates.| Product Name | MIF Antibody (FL-115) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor å (TNFå), interleukin-1 (IL-1) and interferon-© (IFN-©). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MIF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-115 of MIF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-115 of MIF of human origin |
| Entrez Gene | 4282 |
|---|
Application
Western blot analysis of MIF expression in AML-193 whole cell lysate.
Application
Western blot analysis of MIF expression in Jurkat whole cell lysate.
Application
Western blot analysis of MIF expression in non-transfected 293T: (A), human MIF transfected 293T: (B) and Y79 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic and membrane staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing cytoplasmic and extracellular localization.
Application
Western blot analysis of MIF expression in Y79 (A), U-937 (B) and HL-60 (C) whole cell lysates.
Application
Western blot analysis of MIF expression in U-937 (A) and HL-60 (B) whole cell lysates.| Application Notes | recommended for detection of MIF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
