Application
Western blot analysis of VEGF-D expression in non-transfected: (A) and mouse VEGF-D transfected: (B) 293T whole cell lysates.Application
Western blot analysis of VEGF-D expression in non-transfected 293T: (A), human VEGF-D transfected 293T: (B) and MCF7 (C) whole cell lysates.Application
Western blot analysis of human recombinant VEGF-D fusion protein.Application
Immunofluorescence staining of methanol-fixed MH-S cells showing cytoplasmic localization.| Product Name | VEGF-D Antibody (H-144) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). Several forms of VEGF have been identified, including VEGF, VEGF-B, VEGF-C and VEGF-D (also designated FIGF). Characteristic of VEGF proteins, the central region of VEGF-D contains eight cysteine residues. These residues are essential for homodimerization. VEGF-D may play a role in tumor progression, as it is induced by c-Fos, which is required for conversion of early stage tumors to malignant tumors. It has been observed that overexpression of VEGF-D induces morphological changes in fibroblasts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor VEGF-D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-354 mapping at the C-terminus of VEGF-D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-354 mapping at the C-terminus of VEGF-D of human origin |
| Entrez Gene | 2277 |
|---|
Application
Western blot analysis of VEGF-D expression in non-transfected: (A) and mouse VEGF-D transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of VEGF-D expression in non-transfected 293T: (A), human VEGF-D transfected 293T: (B) and MCF7 (C) whole cell lysates.
Application
Western blot analysis of human recombinant VEGF-D fusion protein.
Application
Immunofluorescence staining of methanol-fixed MH-S cells showing cytoplasmic localization.| Application Notes | recommended for detection of precursor VEGF-D of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
