Application
Western blot analysis of casein kinase Iδ expression in non-transfected: (A) and mouse casein kinase Iδ transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | casein kinase Iδ Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iå, ©, ∂ and é, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. Casein kinase II is usually expressed as a tetrameric complex consisting of either an å2∫2 or an åå'∫2 structure. The åcatalytic subunit is stimulated by the ∫ regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in both the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of casein kinase I&delta of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 296-355 mapping near the C-terminus of casein kinase I&delta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 296-355 mapping near the C-terminus of casein kinase Iδ of human origin |
| Entrez Gene | 1453 |
|---|
Application
Western blot analysis of casein kinase Iδ expression in non-transfected: (A) and mouse casein kinase Iδ transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of casein kinase I&delta of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
