Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of Wee 1 expression in non-transfected: (A) and mouse Wee 1 transfected: (B) 293T whole cell lysates and K-562 nuclear extract (C).Application
Western blot analysis of Wee 1 in Jurkat (A) and K-562 (B) nuclear extracts and BJAB (C) whole cell lysate.| Product Name | Wee 1 Antibody (C-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 protein, Wee 1 Hu, encodes a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Activity of both Wee 1 Hu and Myt 1 is regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Wee 1 p98 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Wee 1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Wee 1 of human origin |
| Entrez Gene | 7465 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Wee 1 expression in non-transfected: (A) and mouse Wee 1 transfected: (B) 293T whole cell lysates and K-562 nuclear extract (C).
Application
Western blot analysis of Wee 1 in Jurkat (A) and K-562 (B) nuclear extracts and BJAB (C) whole cell lysate.| Application Notes | recommended for detection of Wee 1 p98 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
