Application
Antibodies tested include p-MEK-1 (B-4): (A,B,C) and MEK-1 (C-18): (D,E,F).Application
Antibodies tested include p-MEK-1 (Ser 298)-R: (A,B) and MEK-1 (C-18): (C,D).Application
Antibodies tested include p-MEK-1 (B-4): (A,B) and MEK-1 (C-18): (C,D).Application
Antibodies tested include MEK-1 (C-18): , MEK kinase-1 (C-22): , HDAC1 (C-19): , HDAC1 (H-11): and HDAC1 (H-11): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Cytoplasmic immunofluorescence staining of methanol-fixed HeLa cells.Application
Western blot analysis of MEK-1 expression in KNRK (A) and CTLL-2 (B) whole cell lysates.| Product Name | MEK-1 Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of MEK-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of MEK-1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of MEK-1 of rat origin |
| Entrez Gene | 5604 |
|---|
Application
Antibodies tested include p-MEK-1 (B-4): (A,B,C) and MEK-1 (C-18): (D,E,F).
Application
Antibodies tested include p-MEK-1 (Ser 298)-R: (A,B) and MEK-1 (C-18): (C,D).
Application
Antibodies tested include p-MEK-1 (B-4): (A,B) and MEK-1 (C-18): (C,D).
Application
Antibodies tested include MEK-1 (C-18): , MEK kinase-1 (C-22): , HDAC1 (C-19): , HDAC1 (H-11): and HDAC1 (H-11): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Cytoplasmic immunofluorescence staining of methanol-fixed HeLa cells.
Application
Western blot analysis of MEK-1 expression in KNRK (A) and CTLL-2 (B) whole cell lysates.| Application Notes | recommended for detection of MEK-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
