Application
Western blot analysis of TBK1 expression in non-transfected: (A) and human TBK1 transfected: (B) 293T whole cell lysates.| Product Name | TBK1 Antibody (M-375) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase å (IKKå), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-å on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKå, IKKß and IKK© (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues. TBK1 was shown to complex with TRAF2 and TANK in the NFkB activation pathway. TBK1 shares homology with IKKå and IKKß in the amino-terminal half, which includes the kinase domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TBK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcineWB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 355-729 mapping at the C-terminus of TBK1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 355-729 mapping at the C-terminus of TBK1 of mouse origin |
| Entrez Gene | 29110 |
|---|
Application
Western blot analysis of TBK1 expression in non-transfected: (A) and human TBK1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TBK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcineWB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
