Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Cytokeratin 8 phosphorylation expression in untreated (A) and EGF treated (B) Hep G2 whole cell lysates.| Product Name | p-Cytokeratin 8 Antibody (Ser 74) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 correlates with malignancy in leukoplakia and carcinomas of the head and neck, and is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy. P-Cytokeratin 8 is phosphorylated at S431. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 74 phosphorylated Cytokeratin 8 of human origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Ser 74 phosphorylated Cytokeratin 8 of human origin by WB, IP and IF |
| Entrez Gene | 3856 |
|---|
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Cytokeratin 8 phosphorylation expression in untreated (A) and EGF treated (B) Hep G2 whole cell lysates.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
