Application
Western blot analysis of Sox-30 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing strong nuclear staining of late spermatids at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Sox-30 expression in SK-N-MC (A), IMR-32 (B) and A-431 (C) nuclear extracts.| Product Name | Sox-30 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sox-30 (SRY (sex determining region Y)-box 30) encodes a member of the Sox (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. Sox-30 is expressed in testis and may act as a transcriptional regulator after forming a protein complex with other proteins. Sox-30 may be involved in the differentiation of developing male germ cells. Two transcript variants encoding distinct isoforms have been identified for the human Sox-30 gene. Sox family transcription factors influence cell differentiation, development and sex determination. Sox-30 contains a unique DNA binding domain, known as the high mobility group (HMG) box, that is related to that of the testis determining gene, SRY. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. Several Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Sox-30 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 454-753 mapping at the C-terminus of Sox-30 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 454-753 mapping at the C-terminus of Sox-30 of human origin |
| Entrez Gene | 11063 |
|---|
Application
Western blot analysis of Sox-30 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing strong nuclear staining of late spermatids at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Sox-30 expression in SK-N-MC (A), IMR-32 (B) and A-431 (C) nuclear extracts.| Application Notes | recommended for detection of Sox-30 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
