Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear and cytoplasmic staining of myocytes.Application
Western blot analysis of B23 expression in HEL 92.1.7 (A), CCRF-CEM (B), HeLa (C), K-562 (D) and DU 145 (E) whole cell lysates.Application
Western blot analysis of B23 expression in 293T (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of B23 expression in non-transfected 293T: (A), human B23 transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.Application
Western blot analysis of B23 expression in non-transfected: (A) and human B23 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of B23 expression in MCP-5 whole cell lysate.Application
Western blot analysis of B23 expression in HEL 92.1.7 (A), LNCaP (B) and HeLa (C) whole cell lysates.| Product Name | B23 Antibody (H-106) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The transport of proteins across the nuclear envelope is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex, and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. For example, two cytosolic factors critically involved in the recognition and docking process are the karyopherin å and karyopherin ∫ proteins. The karyopherin holoenzyme is a heterodimer of å and ∫ subunits. The nuclear protein B23 (also referred to as nucleophosmin) is involved in ribosomal assembly and rRNA transport. B23 is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of B23 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 174-280 mapping at the C-terminus of B23 nucleolar phosphoprotein of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 174-280 mapping at the C-terminus of B23 nucleolar phosphoprotein of human origin |
| Entrez Gene | 4869 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear and cytoplasmic staining of myocytes.
Application
Western blot analysis of B23 expression in HEL 92.1.7 (A), CCRF-CEM (B), HeLa (C), K-562 (D) and DU 145 (E) whole cell lysates.
Application
Western blot analysis of B23 expression in 293T (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of B23 expression in non-transfected 293T: (A), human B23 transfected 293T: (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Western blot analysis of B23 expression in non-transfected: (A) and human B23 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of B23 expression in MCP-5 whole cell lysate.
Application
Western blot analysis of B23 expression in HEL 92.1.7 (A), LNCaP (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of B23 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
