Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of cells in germinal and non-germinal centers.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of RecQL1 expression in non-transfected: (A) and mouse RecQL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RecQL1 expression in NIH/3T3 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of RecQL1 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).| Product Name | RecQL1 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In humans, the RecQ helicase family includes WRN, BLM, RecQL1, RecQL4 and RecQL5 proteins, all of which contain a conserved helicase domain. WRN and BLM have been demonstrated to be the responsible genes in Werner and Bloom syndromes, respectively. RecQL1 and RecQL5 also belong to the human RecQ helicase family, but their correlation with genetic disorders, if any, is unknown. The gene encoding human RecQL4, which maps to chromosome 8q24.3, is believed to be the gene responsible for the development of Rothmund-Thomson syndrome (RTS). The levels of WRN, BLM, RecQL1, 4 and 5 are differentially upregulated to guarantee genomic stability in cells that are transformed or actively proliferating. In humans, RecQL1 and RecQL5 map to chromosome 12p12.1 and 17q25.1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RecQL1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-110 of RecQL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-110 of RecQL1 of human origin |
| Entrez Gene | 5965 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of cells in germinal and non-germinal centers.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of RecQL1 expression in non-transfected: (A) and mouse RecQL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RecQL1 expression in NIH/3T3 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of RecQL1 expression in HeLa whole cell lysate (A) and HeLa nuclear extract (B).| Application Notes | recommended for detection of RecQL1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
