Application
Western blot analysis of Ah Receptor expression in non-transfected: (A) and mouse Ah Receptor transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.Application
Western blot analysis of Ah Receptor expression in PC-3 whole cell lysate.| Product Name | Ah Receptor Antibody (H-211) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the prototype for a family of toxic halogenated aromatic compounds that are thought to cause adverse reproductive, immunologic and metabolic effects. Many biological responses to TCDD are mediated through ligand binding to the aromatic hydrocarbon (Ah) receptor, also known as AhR. Ah Receptor is a ligand dependent transcription factor that interacts with specific DNA sequences, termed xenobiotic responsive elements (XREs), and that lies upstream of TCDD-inducible genes. Upon binding to the ligand, Ah Receptor binds to the Ah Receptor nuclear translocator (Arnt), and the complex is translocated from the cytoplasm to the nucleus. Arnt is required for Ah R eceptor to bind to XRE. Ah Receptor and Arnt are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Ah Receptor of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 637-848 of Ah Receptor of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 637-848 of Ah Receptor of human origin |
| Entrez Gene | 196 |
|---|
Application
Western blot analysis of Ah Receptor expression in non-transfected: (A) and mouse Ah Receptor transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization.
Application
Western blot analysis of Ah Receptor expression in PC-3 whole cell lysate.| Application Notes | recommended for detection of Ah Receptor of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
