Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Id2 expression in non-transfected: (A) and mouse Id2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Id2 expression in non-transfected 293T: (A), mouse Id2 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.Application
Western blot analysis of Id2 expression in SK-N-MC whole cell lysate.| Product Name | Id2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 (1–3), Id2 (4), Id3 and Id4 (5). They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins (1–5). Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation (6,7). It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Id2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Id2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Id2 of mouse origin |
| Entrez Gene | 3398 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Id2 expression in non-transfected: (A) and mouse Id2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Id2 expression in non-transfected 293T: (A), mouse Id2 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.
Application
Western blot analysis of Id2 expression in SK-N-MC whole cell lysate.| Application Notes | recommended for detection of Id2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
