Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.Application
Western blot analysis of HDAC7 expression in non-transfected: (A) and human HDAC7 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HDAC7 expression in K-562 nuclear extract (A) and SUP-T1 whole cell lysate (B).Application
Western blot analysis of HDAC7 expression in non-transfected: (A) and human HDAC7 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of HDAC7 expression in non-transfected control (A) and HDAC7 siRNA transfected (B) HeLa cells. Blot probed with HDAC4/5/7 (H-273): . a-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of HDAC7 expression in NIH/3T3 whole cell lysate (A) and NIH/3T3 nuclear extract (B).Application
Western blot analysis of HDAC7 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of human recombinant HDAC7 fusion protein.| Product Name | HDAC4/5/7 Antibody (H-273) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-7, have been identified as histone deacetylases. Mammalian HDAC7 interacts with the adaptor mSin3A. The interaction of HDAC7 with mSin3A suggests the association of multiple repression complexes of transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of HDAC4, HDAC5 and HDAC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-435 mapping near the N-terminus of HDAC7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-435 mapping near the N-terminus of HDAC7 of human origin |
| Entrez Gene | 51564 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal and glial cells.
Application
Western blot analysis of HDAC7 expression in non-transfected: (A) and human HDAC7 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HDAC7 expression in K-562 nuclear extract (A) and SUP-T1 whole cell lysate (B).
Application
Western blot analysis of HDAC7 expression in non-transfected: (A) and human HDAC7 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of HDAC7 expression in non-transfected control (A) and HDAC7 siRNA transfected (B) HeLa cells. Blot probed with HDAC4/5/7 (H-273): . a-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of HDAC7 expression in NIH/3T3 whole cell lysate (A) and NIH/3T3 nuclear extract (B).
Application
Western blot analysis of HDAC7 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of human recombinant HDAC7 fusion protein.| Application Notes | recommended for detection of HDAC4, HDAC5 and HDAC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
