Application
Western blot analysis of C/EBP ε expression in non-transfected: (A) and human C/EBP ε transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C/EBP expression in K-562 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear staining of bone marrow poietic cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization in selected cells.| Product Name | C/EBP ε Antibody (C-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The transcription factor C/EBP å (CCAAT-enhancer binding protein) is a heat-stable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP å regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP å uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP å appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP ∫, C/EBP ©, C/EBP ∂ and C/EBP e, all of which exhibit similar DNA-binding specificities and affinities to C/EBP å. Furthermore, C/EBP ∫ and C/EBP ∂ readily form heterodimers both with each other as well as with C/EBP å. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of C/EBP &epsilon of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of C/EBP &epsilon of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of C/EBP ε of rat origin |
| Entrez Gene | 1053 |
|---|
Application
Western blot analysis of C/EBP ε expression in non-transfected: (A) and human C/EBP ε transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C/EBP expression in K-562 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear staining of bone marrow poietic cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear localization in selected cells.| Application Notes | recommended for detection of C/EBP &epsilon of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
