Application
Western blot analysis of cytochrome b expression in Hep G2 whole cell lysate.Application
Western blot analysis of human recombinant cytochrome b fusion protein.| Product Name | cytochrome b Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cytochrome b is a component of the ubiquinol-cytochrome c reductase complex, which is a respiratory chain that generates an electrochemical potential, coupled to ATP synthesis. The principal components of the b-c1 complex are cytochrome b, cytochrome c1, and the rieske protein. Cytochrome b posesses two heme groups, which are not covalently attached to the protein. Mutations in cytochrome b are associated with Leber’s hereditary optic neuropathy and with myopathy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cytochrome b of multiple species, including mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 18-300 mapping at the N-terminus of cytochrome b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 18-300 mapping at the N-terminus of cytochrome b of human origin |
| Entrez Gene | 4519 |
|---|
Application
Western blot analysis of cytochrome b expression in Hep G2 whole cell lysate.
Application
Western blot analysis of human recombinant cytochrome b fusion protein.| Application Notes | recommended for detection of cytochrome b of multiple species, including mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
