Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of TANK expression in Ramos whole cell lysate.Application
Western blot analysis of TANK expression in non-transfected 293T: (A), mouse TANK transfected 293T: (B) and HCT 116 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TANK expression in Ramos (A) and Jurkat (B) whole cell lysates.| Product Name | TANK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The tumor necrosis factor (TNF) receptor superfamily is composed of several type I integral membrane glycoproteins that exhibit homology in their cysteine-rich extracellular domains. Members of this family include TNF-RI and -RII, FAS, OX40, CD27, CD30 and CD40. Ligands for these receptors can be small, secreted proteins such as TNF, or type II integral membrane proteins, such as the CD40 ligand, CD40L. While the signal transduction mechanism of the TNF receptor superfamily is poorly understood, stimulation of cells with either TNF or soluble CD40L has been shown to induce the nuclear translocation of NFkB. Members of the TRAF family associate with activated TNF-R and CD40 and have been implicated in this process. The discovery of a novel protein, designated TANK, has shed light on the means by which TRAF activation of NFkB occurs. TANK is not only capable of binding to all three TRAFs, but also of synergizing with TRAF2 to activate the NFkB signaling cascad. TANK contains a regulatory carboxy terminal domain that maintains its inactivity in unstimulated cells. Upon its association with TRAF2, the inhibitory effect of this domain is overcome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TANK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 126-425 mapping at the C-terminus of TANK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 126-425 mapping at the C-terminus of TANK of human origin |
| Entrez Gene | 10010 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of TANK expression in Ramos whole cell lysate.
Application
Western blot analysis of TANK expression in non-transfected 293T: (A), mouse TANK transfected 293T: (B) and HCT 116 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human parathyroid gland tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TANK expression in Ramos (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of TANK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
