Application
Western blot analysis of MLTK expression in HeLa whole cell lysate.| Product Name | MLTK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MLTK (mitogen-activated protein kinase kinase kinase MLT, leucine zipper- and sterile a motif-containing kinase, HCCS-4) is an 800 amino acid protein encoded by the human gene ZAK. MLTK belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily and contains one protein kinase domain and one SAM (sterile a motif) domain. MLTK is a cytoplasmic protein found as a homodimer. It will translocate to the nucleus upon ultraviolet B irradiation. It is a stress-activated component of a protein kinase signal transduction cascade and helps regulates the JNK and p38 pathways. MLTK also has a role in the regulation of S and G2 cell cycle checkpoint by direct phosphorylation of Chk2. Isoform 1 causes cell shrinkage and disruption of Actin stress fibers and may have a role in neoplastic cell transformation and cancer development. Isoform 1 also phosphorylates Histone H3 at Ser 28. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MLTK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 176-475 mapping within an internal region of MLTK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 176-475 mapping within an internal region of MLTK of human origin |
| Entrez Gene | 51776 |
|---|
Application
Western blot analysis of MLTK expression in HeLa whole cell lysate.| Application Notes | recommended for detection of MLTK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
