Application
Antibodies tested include p-GABAB R2 (Ser 783): (A, B) and GABAB R2 (E-16): (C, D).Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.Application
Western blot analysis of GABAB R2 phosphorylation in 293T whole cell lysate.Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat synaptic membrane tissue extracts.| Product Name | p-GABAB R2 Antibody (Ser 783) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | GAD-65 and GAD-67, glutamate decarboxylases, catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA (gamma-aminobutyric acid) acts as the main inhibitory transmitter by increasing a Cl-- conductance that inhibits neuronal firing. GABA activates both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Members of the GABAA receptor family include GABAA Rå1-6, GABAA R ∫1-3, GABAA R©1-3, GABAA R∂, GABAA Ré, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1å and GABAB R1∫. PKA phosphorylates GABABR2 at Ser 892. This phosphorylation appears to enhance the membrane stability of GABAB R2. Mouse, rat and human GABAB R2 are phosphorylated on Ser 783 by AMPK∫1, which stabilizes GABAB b activation of inwardly rectifying K+ channels and decreases synaptic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 783 phosphorylated GABAB R2 of mouse and rat origin and correspondingly Ser 784 phosphorylated GABAB R2 of human origin by WB IP and IF also reactive with additional species including bovine |
|---|---|
| Tissue Specificity | recommended for detection of Ser 783 phosphorylated GABAB R2 of mouse and rat origin and correspondingly Ser 784 phosphorylated GABAB R2 of human origin by WB, IP and IF; also reactive with additional species, including bovine |
| Entrez Gene | 9568 |
|---|
Application
Antibodies tested include p-GABAB R2 (Ser 783): (A, B) and GABAB R2 (E-16): (C, D).
Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.
Application
Western blot analysis of GABAB R2 phosphorylation in 293T whole cell lysate.
Application
Western blot analysis of GABAB R2 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat synaptic membrane tissue extracts.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
