Application
Western blot analysis of ARIH1 expression in non-transfected: (A) and human ARIH1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARIH1 expression in Jurkat whole cell lysate.| Product Name | ARIH1 Antibody (Q-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ARIH1 (ariadne homolog), also known as ubiquitin conjugating enzyme E2 binding protein 1, ARI, HARI, HHARI (human homolog of Drosophila ariadne), MOP-6 (monocyte protein 6) or UBCH7BP (UBCH7 binding protein), is a 557 amino acid cytoplasmic protein. Expressed in a wide variety of tissues, ARIH1 contains two RING-type zinc fingers and one IBR (in-between RING fingers)-type domain. ARIH1 is believed to be involved in protein degradation and protein translation. ARIH1 interacts with UBCH7 and is thought to function as an E3 ubiquitin-protein ligase (or as a component of an E3 complex) that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme (UBCH7) and transfers that ubiquitin residue to substrates targeted for degradation. Specifically, ARIH1 interacts with and polyubiquitylates eIF4E2, thereby targeting it for proteasomal degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of ARIH1 of mouse, human, canine and zebrafish origin by WB, IP and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ARIH1 of human origin |
|---|---|
| Tissue Specificity | raised against ARIH1 of human origin |
| Entrez Gene | 25820 |
|---|
Application
Western blot analysis of ARIH1 expression in non-transfected: (A) and human ARIH1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARIH1 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of ARIH1 of mouse, human, canine and zebrafish origin by WB, IP and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
