Application
Western blot analysis of phosphorylated RelB expression in untreated (A) and EGF-treated (B) A431 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-RelB Antibody (Ser 552) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The NF˚B transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A second protein designated p52 (previously referred to as p49) has been identified that can act as an alternative NF˚B subunit. Rel B does not bind with high affinity to NFkB sites, but heterodimers between Rel B and p50 bind with an affinity comparable to that of p50 NF˚B homodimers. However, Rel B/p50 heterodimers, in contrast to NF˚B heterodimers, transactivates transcription of promotors containing ˚B binding sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 552 phosphorylated RelB of mouse origin and correspondingly phosphorylated Ser 573 of human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 552 of RelB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 552 of RelB of human origin |
| Entrez Gene | 5971 |
|---|
Application
Western blot analysis of phosphorylated RelB expression in untreated (A) and EGF-treated (B) A431 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 552 phosphorylated RelB of mouse origin and correspondingly phosphorylated Ser 573 of human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
