Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of eIF4AIII expression in non-transfected 293T: (A), mouse eIF4AIII transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of elF4AIII expression in MCF7 (A) and HeLa (B) nuclear extracts.| Product Name | eIF4AIII Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The eukaryotic translation factor 4A (eIF4A) is a member of DEA(D/H)-box RNA helicase family that couples ATP hydrolysis to RNA binding and duplex separation. eIF4A participates in the initiation of translation by unwinding secondary structure in the 5'-untranslated region of mRNAs and facilitating scanning by the 40 S ribosomal subunit for the initiation codon. eIF4AIII is a component of the exon junction complex (EJC) that assembles near exon-exon junctions of mRNAs as a result of splicing. eIF4AIII, but not eIF4AI or eIF4AII, preferentially associates with spliced mRNA. eIF4AIII is found in the nucleus whereas eIF4AI and eIF4AII are found in the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF4AIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of eIF4AIII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of eIF4AIII of human origin |
| Entrez Gene | 9775 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of eIF4AIII expression in non-transfected 293T: (A), mouse eIF4AIII transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of elF4AIII expression in MCF7 (A) and HeLa (B) nuclear extracts.| Application Notes | recommended for detection of eIF4AIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
