Application
Western blot analysis of EMAP II expression in non-transfected: (A) and human EMAP II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of EMAP II expression in non-transfected 293T: (A), mouse EMAP II transfected 293T: (B) and THP-1 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of glial and neuronal cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of EMAP II expression in UV treated HeLa (A), T98G (B) and HT-1080 (C) whole cell lysates.| Product Name | EMAP II Antibody (H-92) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Endothelial monocyte-activating polypeptide (EMAP II), also known as small inducible cytokine subfamily E, member 1 (SCYE1), is a chemoattractant cytokine for monocytes and granulocytes that is inducible by apoptosis. TNFå treatment of murine meth A fibrosarcomas and B16 melanomas upregulates EMAP II mRNA production. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. EMAP II mRNA translates as a precursor protein, proEMAP II, which undergoes proteolysis to become the mature, biologically active cytokine. ProEMAP II may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature EMAP II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-312 mapping at the C-terminus of EMAP II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-312 mapping at the C-terminus of EMAP II of human origin |
| Entrez Gene | 9255 |
|---|
Application
Western blot analysis of EMAP II expression in non-transfected: (A) and human EMAP II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of EMAP II expression in non-transfected 293T: (A), mouse EMAP II transfected 293T: (B) and THP-1 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of glial and neuronal cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of EMAP II expression in UV treated HeLa (A), T98G (B) and HT-1080 (C) whole cell lysates.| Application Notes | recommended for detection of precursor and mature EMAP II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
