Application
Western blot analysis of CHMP4A expression in non-transfected: (A) and human CHMP4A transfected: (B) 293T whole cell lysates.| Product Name | CHMP4A Antibody (H-52) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The charged multivesicular body proteins or chromatin modifying proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs) and are required for the formation of these bodies. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins via the association of CHMP4 and a protein recruited by HIV-1 p6, called PDCD61P, which is present in viral Gag assembly and budding. CHMP4 is strongly expressed in kidney, liver, heart and skeletal muscle and to a lesser degree in lung, brain and pancreas. Three CHMP4 isoforms, encoded by distinct genes, are designated CHMP4A, CHMP4B and CHMP4C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CHMP4A of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 171-222 mapping at the C-terminus of CHMP4A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 171-222 mapping at the C-terminus of CHMP4A of human origin |
| Entrez Gene | 29082 |
|---|
Application
Western blot analysis of CHMP4A expression in non-transfected: (A) and human CHMP4A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CHMP4A of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
