Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.Application
Western blot analysis of GDF-15 expression in LNCaP whole cell lysate.| Product Name | GDF-15 Antibody (FL-308) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Growth differentiation factor 15 (GDF-15), also known as PDF, MIC-1, PLAB, NAG-1 or PTGF-∫, is a member of the transforming growth factor beta (TGF-beta) superfamily. Synthesized intracellularly, the protein is secreted as a dimer linked by disulfide bonds. Epithelial cells and macrophages are the sites of strongest GDF-15 expression, although it is widely expressed in adult tissue. In the brain, GDF-15 expression occurs in the choroid plexus, from which the protein is secreted into the cerebrospinal fluid. The gene for GDF-15 is responsive to p53 tumor suppressor protein, and in cultured cerebellar granule neurons GDF-15 can prevent cell death by the activation of Akt and inhibition of ERK. GDF-15 acts as a trophic factor for certain classes of neurons, promoting cell survival and differentiation. Overexpression of GDF-15 occurs in prostate cancer, and may be a means of diagnosis. In the uterus, GDF-15 may work to suppress maternally derived proinflammatory cytokines, thereby promoting fetal survival. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature GDF-15 of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-308 representing full length GDF-15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-308 representing full length GDF-15 of human origin |
| Entrez Gene | 9518 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells.
Application
Western blot analysis of GDF-15 expression in LNCaP whole cell lysate.| Application Notes | recommended for detection of precursor and mature GDF-15 of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
