Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and human BMAL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and mouse BMAL1 transfected: (B) 293T whole cell lysates and mouse liver nuclear extract (C).Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and mouse BMAL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and human BMAL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BMAL1 expression in 3T3-L1 whole cell lysate.| Product Name | BMAL1 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | AhR, Arnt 1, Arnt 2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (brain and muscle Arnt-like protein 1), also designated Arnt3, TIC, JAP3 or MOP3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of BMAL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 441-610 mapping near the C-terminus of BMAL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 441-610 mapping near the C-terminus of BMAL1 of human origin |
| Entrez Gene | 406 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and human BMAL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and mouse BMAL1 transfected: (B) 293T whole cell lysates and mouse liver nuclear extract (C).
Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and mouse BMAL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BMAL1 expression in non-transfected: (A) and human BMAL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BMAL1 expression in 3T3-L1 whole cell lysate.| Application Notes | recommended for detection of BMAL1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
