Application
Western blot analysis of toll expression in Schneider's Drosophila whole cell lysate.| Product Name | toll Antibody (d-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Drosophila melanogaster is a proven and effective model for studying developmental and cellular processes common to higher eukaryotes. Approximately 13,600 genes have been elucidated from more than 120 megabases of euchromatin, and they are organized among the chromosomes 2, 3, 4, X and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes can be categorized based on the type of protein for which they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated) or other functional proteins (2). Many of the genes expressed in Drosophila are structurally and functionally similar across species, as are the pathways involved in transducing intracellular signaling. Toll (dToll), whose mammalian homolog is the Interleukin-1 (IL-1) receptor, contributes to proper embryonic dorsal-ventral patterning during development and mediates immune response through a Toll-Cactus-Dorsal pathway (3-5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of toll of Drosophila origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-330 mapping within an N-terminal extracellular domain of toll of Drosophila melanogaster origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-330 mapping within an N-terminal extracellular domain of toll of Drosophila melanogaster origin |
Application
Western blot analysis of toll expression in Schneider's Drosophila whole cell lysate.| Application Notes | recommended for detection of toll of Drosophila origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
