| Product Name | p-Rad17 Antibody (Ser 656) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA damage results in the arrest of cell cycle progression, allowing the damaged DNA to be repaired prior to replication. Checkpoints exist at several cell cycle phase transitions to maintain this genetic integrity. Rad9, Rad17, Rad24 and Mec3 are involved in activating the G1 and G2 checkpoints. Pol2 (also known as Dun2), encoding the catalytic subunit of DNA polymerase epsilon, plays a role in activating the S phase checkpoint. The protein kinase Rad53 (also designated Spk1, Mec2 or Sad1) is essential for both G2 and S phase arrest. Activation of Rad53 is regulated by Mec1 (also known as Esr1 and Sad3), a homolog of the human ATM protein. Pds1 and Mad2 both regulate checkpoints associated with incomplete spindle replication. Dun1, another protein kinase, plays a role in transducing the DNA damage signal. The phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Ser 656 phosphorylated Rad17 of human origin, Ser 657 phosphorylated Rad17 of mouse origin and correspondingly phosphorylated Rad17 of rat, equine, canine, bovine and porcine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 656 of Rad17 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 656 of Rad17 of human origin |
| Entrez Gene | 5884 |
|---|
| Application Notes | recommended for detection of Ser 656 phosphorylated Rad17 of human origin, Ser 657 phosphorylated Rad17 of mouse origin and correspondingly phosphorylated Rad17 of rat, equine, canine, bovine and porcine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
