Application
Western blot analysis of AAT expression in non-transfected: (A) and human AAT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AAT expression in non-transfected: (A) and human AAT transfected: (B) 293 whole cell lysates.| Product Name | AAT Antibody (H-203) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AAT of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 216-418 mapping at the C-terminus of AAT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 216-418 mapping at the C-terminus of AAT of human origin |
| Entrez Gene | 5265 |
|---|
Application
Western blot analysis of AAT expression in non-transfected: (A) and human AAT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AAT expression in non-transfected: (A) and human AAT transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of AAT of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
