IHC
(A, B) Representative micrographs of 4-HNE (A) and 8-OHdG (B) staining in spinal cord tissues of different groups.| Product Name | 8-OHdG Antibody (J-1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | DNA or RNA damage can hinder the ability of a cell to carry out its function and can significantly increase the likelihood of tumor formation. One of the causes of damaged DNA and RNA is oxidation of the bases. 8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine (8-OHdG) and 8-hydroxyguanosine are all markers of oxidative damage to RNA and DNA. 8-hydroxy-2'-deoxyguanosine is produced by reactive oxygen and nitrogen species, including hydroxyl radical and peroxynitrite. 8-Hydroxyguanine is one of the major base lesions involved in mutagenesis and is caused by ionizing radiation and radiomimetic agents. 8-hydroxy-guanosine induces a transversion of G to T in DNA, which may be mutagenic. Markers of DNA and RNA damage are useful research tools when studying the effects of this type of damage. |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIFIHC |
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of 8-OHdG modified proteins of nsr origin by WB IF and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of 8-OHdG modified proteins of nsr origin by WB, IF and ELISA |
IHC
(A, B) Representative micrographs of 4-HNE (A) and 8-OHdG (B) staining in spinal cord tissues of different groups.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
