| Product Name | ATP7A Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood. Mutations in the ATP7B gene lead to the autosomal recessive disorder, Wilson disease, characterized by neurological symptoms and hepatic damage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ATP7A (also known as Copper-transporting ATPase 1) isoforms 1, 2, 4, 5 and to a lesser extent isoforms 3 and 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-180 mapping within an N-terminal cytoplasmic domain of ATP7A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-180 mapping within an N-terminal cytoplasmic domain of ATP7A of human origin |
| Entrez Gene | 538 |
|---|
| Application Notes | recommended for detection of ATP7A (also known as Copper-transporting ATPase 1) isoforms 1, 2, 4, 5 and to a lesser extent isoforms 3 and 6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
