Application
Western blot analysis of SNX9 expression in non-transfected: (A) and human SNX9 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SNX9 expression in non-transfected 293T: (A), human SNX9 transfected 293T: (B) and 3T3-L1 (C) whole cell lysates.| Product Name | SNX9 Antibody (H-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sorting nexin (SNX) proteins are members of a large family of hydrophilic PX (phospholipid-binding motif) domain-containing proteins that interact with a variety of receptor types. SNXs are widely expressed, although the tissue distribution of each SNX mRNA varies. The ability of SNXs to bind specific phospholipids, as well as their tendency to form protein-protein complexes, suggests a role for these proteins in cellular membrane trafficking and protein sorting. SNXs may also function specifically in pro-degradative sorting, internalization, endosomal recycling or simply in endosomal sorting. SNXs partially associate with cellular membranes, despite their hydrophilic nature. SNX9 resides in the cytosol where it influences the processing and trafficking of insulin receptors. The enzyme aldolase binds to and inactivates SNX9. Phosphorylation of SNX9 releases aldolase and frees SNX9 to recruit and activate Dynamin II, a neuronal phosphoprotein and a GTPase enzyme which mediates late stages of endocytosis in both neural and non-neural cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SNX9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 391-436 mapping within an internal region of SNX9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 391-436 mapping within an internal region of SNX9 of human origin |
| Entrez Gene | 51429 |
|---|
Application
Western blot analysis of SNX9 expression in non-transfected: (A) and human SNX9 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SNX9 expression in non-transfected 293T: (A), human SNX9 transfected 293T: (B) and 3T3-L1 (C) whole cell lysates.| Application Notes | recommended for detection of SNX9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
