Application
Western blot analysis of Ndfip2 expression in non-transfected: (A) and mouse Ndfip2 transfected: (B) 293T whole cell lysates.| Product Name | Ndfip2 Antibody (P-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ndfip2 (NEDD4 family interacting protein 2), also known as N4WBP5A, is a 336 amino acid protein that localizes to the membrane of both the endosome and the golgi apparatus. Expressed in kidney, heart, brain, lung, liver, placenta and skeletal muscle, Ndfip2 interacts with NEDD4 and NEDD4-L and is thought to be involved in endocytosis and in the NF˚B and MAPK signaling pathways. Additionally, Ndfip2 may function as an adaptor protein that may recruit NEDD4 ubiquitin-protein ligases to protein trafficking machinery in the golgi. Ndfip2 is activated by T cells and may be ubiquitinated by NEDD4 or NEDD4-L, an event that does not affect Ndfip2 function. The gene encoding Ndfip2 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Ndfip2 of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of Ndfip2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of Ndfip2 of human origin |
| Entrez Gene | 54602 |
|---|
Application
Western blot analysis of Ndfip2 expression in non-transfected: (A) and mouse Ndfip2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ndfip2 of mouse, rat and human origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
