Application
Western blot analysis of PIS1 expression in non-transfected: (A) and mouse PIS1 transfected: (B) 293T whole cell lysates.| Product Name | PIS1 Antibody (Q-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PIS1, also known as CDIPT (CDP-diacylglycerol--inositol 3-phosphatidyltransferase) or PIS (phosphatidylinositol synthase), is a 213 amino acid multi-pass membrane protein that belongs to the CDP-alcohol phosphatidyltransferase class-I family and exists as two alternatively spliced isoforms. Localizing to Golgi apparatus and the cytoplasmic side of endoplasmic reticulum, PIS1 is widely expressed, with highest expression in adult liver and skeletal muscle. PIS1 catalyzes the biosynthesis of phosphatidylinositol (PtdIns), as well as the PtdIns-inositol exchange reaction, which is due to the reverse reaction of PtdIns synthase and is CMP-dependent. PIS1 may also reduce excessive cellular PtdIns. Highly conserved in yeast and mammals, PIS1 is encoded by a gene that maps to human chromosome 16p11.2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PIS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PIS1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PIS1 of human origin |
| Entrez Gene | 10423 |
|---|
Application
Western blot analysis of PIS1 expression in non-transfected: (A) and mouse PIS1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PIS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
