Application
Western blot analysis of phosphorylated eIF4G expression in untreated (A) and PMA-treated (B) 293 cell extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Product Name | p-eIF4G Antibody (Ser 1232) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures (1,2). eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis (3,4). eIF4A acts as an ATP-dependent RNA helicase (5,6). eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex (7,8). The phosphorylation of human eIF4G is stimulated by Insulin, which targets specific serine resudues, such as Ser 1232, for phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 1232 phosphorylated eIF4G of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Ser 1232 phosphorylated eIF4G of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 1981 |
|---|
Application
Western blot analysis of phosphorylated eIF4G expression in untreated (A) and PMA-treated (B) 293 cell extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
