Application
Western blot analysis of GlyRS expression in non-transfected: (A) and mouse GlyRS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GlyRS expression in HeLa (A) and SK-N-MC (B) whole cell lysates.Application
Western blot analysis of GlyRS expression in Jurkat whole cell lysate.| Product Name | GlyRS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Proteins belonging to this family function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. GlyRS (Glycyl-tRNA synthetase), also known as Glycine-tRNA ligase, is a 739 amino acid class II synthetase that is widely expressed, including in the brain and spinal cord. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Tooth disease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also the cause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distal sensory involvement is less severe in HMN5 patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GlyRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 440-732 mapping at the C-terminus of GlyRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 440-732 mapping at the C-terminus of GlyRS of human origin |
| Entrez Gene | 2617 |
|---|
Application
Western blot analysis of GlyRS expression in non-transfected: (A) and mouse GlyRS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GlyRS expression in HeLa (A) and SK-N-MC (B) whole cell lysates.
Application
Western blot analysis of GlyRS expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of GlyRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
