Application
Immuno?peroxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing membrane localization.| Product Name | L-type Ca++ CP α1S Antibody (H-263) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Voltage-dependent Ca2+ channels mediate Ca2+ entry into excitable cells in response to membrane depolarization, and they are involved in a variety of Ca2+-dependent processes, including muscle contraction, hormone or neurotransmitter release and gene expression (1-3). Calcium channels are highly diverse, multimeric complexes composed of an å-1 subunit, an intracellular ∫-subunit, a disulfide linked å-2/∂ subunit and a transmembrane ©-subunit (1,4). Ca2+ currents are characterized on the basis of their biophysical and pharmacologic properties and include L-, N-, T-, P-, Q-, and R- types (1-3). L-type Ca2+ currents initiate muscle contraction, endocrine secretion, and gene transcription, and can be regulated through second-messenger activated protein phosphorylation pathways (1). L-type calcium channels may form macromolecular signaling complexes with G protein-coupled receptors, thereby enhancing the selectivity of regulating specific targets (5). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of L-type calcium channel &alpha1S of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1611-1873 of L-type calcium channel &alpha 1S of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1611-1873 of L-type calcium channel α1S of human origin |
| Entrez Gene | 779 |
|---|
Application
Immuno?peroxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing membrane localization.| Application Notes | recommended for detection of L-type calcium channel &alpha1S of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
