Application
Western blot analysis of PUSL1 expression in non-transfected: (A) and human PUSL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PUSL1 expression in GA-10 whole cell lysate.| Product Name | PUSL1 Antibody (T-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PUSL1 (tRNA pseudouridylate synthase-like 1) is a 303 amino acid protein that belongs to the tRNA pseudouridine synthase truA family. Existing as two alternatively spliced isoforms, the PUSL1 gene is conserved in dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A.thaliana and rice, and maps to human chromosome 1p36.33. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are all associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. Aberrations in chromosome 1 are found in a variety of cancers including head and neck cancer, malignant melanoma and multiple myeloma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PUSL1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PUSL1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PUSL1 of human origin |
| Entrez Gene | 126789 |
|---|
Application
Western blot analysis of PUSL1 expression in non-transfected: (A) and human PUSL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PUSL1 expression in GA-10 whole cell lysate.| Application Notes | recommended for detection of PUSL1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
