Application
Western blot analysis of NANS expression in non-transfected: (A) and mouse NANS transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NANS expression in non-transfected: (A) and mouse NANS transfected: (B) 293T whole cell lysates.| Product Name | NANS Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sialic acids are a family of 9-carbon 2-keto-3-deoxy sugars that are found on the ends of glycoproteins and glycolipids and play important roles in recognition events within the cell. NANS (N-acetylneuraminic acid synthase), also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Human NANS shares 36% identity with the E. coli protein neuB, suggesting a conserved function between species. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NANS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NANS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NANS of human origin |
| Entrez Gene | 54187 |
|---|
Application
Western blot analysis of NANS expression in non-transfected: (A) and mouse NANS transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NANS expression in non-transfected: (A) and mouse NANS transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of NANS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
