Application
Western blot analysis of SUV39H2 expression in non-transfected control (A) and SUV39H2 siRNA transfected (B) HeLa cell lysates. Kindly provided by The Dr. Michael Rosenfeld Laboratory, University of California San Diego.Application
Western blot analysis of SUV39H2 expression in non-transfected: (A) and human SUV39H2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SUV39H2 expression in HL-60 whole cell lysate.Application
Western blot analysis of SUV39H2 expression in 293 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Product Name | SUV39H2 Antibody (Z-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Distinct modifications of histone tails, such as acetylation, phosphorylation and methylation, regulate nuclear processes by organizing chromatin into higher order structures. Higher order chromatin influences chromosome function and epigenetic gene regulation. SUV39H2 (suppressor of variegation 3-9 homolog 2), also known as KMT1B or Histone H3-K9 methyltransferase 2, is a 410 amino acid protein that localizes to the centromere and contains one SET domain, one pre-SET domain, one post-SET domain and one chromo domain. Expressed at high levels in adult testis, SUV39H2 functions as a histone methyltransferase that trimethylates the Lys-9 residue of Histone H3, thereby playing an essential role in establishing constitutive heterochromatin at pericentric and telomere regions. SUV39H2 conveys its enzymatic activity via its multiple catalytic domains, which are necessary for both stable binding of SUV39H2 to chromatin and for SUV39H2 methyltransferase activity. Multiple isoforms of SUV39H2 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| See 1 citations for SUV39H2Antibody (Z-23) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SUV39H2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SUV39H2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 79723 |
|---|
Application
Western blot analysis of SUV39H2 expression in non-transfected control (A) and SUV39H2 siRNA transfected (B) HeLa cell lysates. Kindly provided by The Dr. Michael Rosenfeld Laboratory, University of California San Diego.
Application
Western blot analysis of SUV39H2 expression in non-transfected: (A) and human SUV39H2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SUV39H2 expression in HL-60 whole cell lysate.
Application
Western blot analysis of SUV39H2 expression in 293 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Application Notes | See 1 citations for SUV39H2Antibody (Z-23) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
